Rapid Batch Method for the Preparation of Fluorescein Isothiocyanate-Conjugated Antibody
نویسندگان
چکیده
منابع مشابه
Rapid batch method for the preparation of fluorescein isothiocyanate-conjugated antibody.
A rapid, reproducible, and relatively simple method for the preparation of large quantities of fluorescein isothiocyanate-conjugated antibody is presented. Tagged globulins with fluorochrome-protein ratios of 1 and 2 were eluted from diethylaminoethyl-cellulose in batch form by using sodium phosphate-buffered 0.1 M and 0.2 M saline, respectively. The intensity and specificity of the fluorescenc...
متن کاملcomparison of the photobleaching and photostability traits of alexa fluor 568- and fluorescein isothiocyanate- conjugated antibody
objective: synthetic fluorescent dyes that are conjugated to antibodies are useful tools to probe molecules. based on dye chemical structures, their photobleaching and photostability indices are quite diverse. it is generally believed that among different fluorescent dyes, alexa fluor family has greater photostability than traditional dyes like fluorescein isothiocyanate (fitc) and cy5. alexa f...
متن کاملComparison of the Photobleaching and Photostability Traits of Alexa Fluor 568- and Fluorescein Isothiocyanate- conjugated Antibody
OBJECTIVE Synthetic fluorescent dyes that are conjugated to antibodies are useful tools to probe molecules. Based on dye chemical structures, their photobleaching and photostability indices are quite diverse. It is generally believed that among different fluorescent dyes, Alexa Fluor family has greater photostability than traditional dyes like fluorescein isothiocyanate (FITC) and Cy5. Alexa Fl...
متن کاملRapid fluorescein and protein assay method for fluorescent-antibody conjugates.
A method is presented for the rapid determination of the fluorescein content, protein content, and fluorescein-to-protein ratio for immune globulin conjugates with fluorescein isothiocyanate as the fluor. This method is based on the absorbance of the fluorescent antibody at those wavelengths primarily associated with the fluorescein and gamma-globulin fractions, and permits these materials to b...
متن کاملساخت آنتی بادی فلورسنت علیه ویروس هاری و بررسی کارایی آن
Abstract Background: Rabies is the most important zoonosis in country. Anti-viral antibody conjugated with Fluorescein Isothiocyanate (FITC), is used for diagnosis of Rabies. We import this reagent from abroad. In this study we aimed to prepare this conjugated fluorescein antibody and assess its utility. Materials and methods: Rabbits were immunized with rabies vaccine, then serum samples ...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1973
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.7.3.366-369.1973